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大学院生・堤 正純さんの論文がFreshwater
Biology誌にオンラインで公開されました。この研究は、山梨大学の岩田智也さんとの共同で行ったものです。<o:p></o:p>
<o:p>
</o:p>
<o:p> </o:p>
著者：Masazumi Tsutsumi, Tomoya
Iwata, Hisaya Kojima, and Manabu Fukui.<o:p></o:p>
題名：Spatiotemporal variations in an assemblage
of closely related planktonic aerobic methanotrophs.<o:p></o:p>
<o:p> </o:p>
＜要旨＞<o:p></o:p>
1. The assemblage of aerobic
methane-oxidising bacteria (MOB) was investigated in different seasons in the
water column of a stratified freshwater lake. Species composition was analysed
by performing denaturing gradient gel electrophoresis (DGGE) of the 16S rRNA
genes and cloning analysis of the pmoA
gene, which encodes the α-subunit of particulate methane monooxygenase. The relative
abundance of MOB to total bacteria was deduced from the copy number of the pmoA gene and 16S rRNA gene
using real-time polymerase chain reaction.<o:p></o:p>
2. The profiles of the DGGE
banding patterns changed with water depth, and these changes correlated with
oxygen concentration and water temperature. The sequences of the DGGE bands
obtained were all associated with the genus Methylobacter.
During the analysis of pmoA
gene, all clones sequenced were that of the Methylobacter/Methylosarcina group. The
relative abundances of pmoA
gene peaked around the oxycline, and small peaks of pmoA gene were also observed near the surface when
peaks of methane were observed at the corresponding depth.<o:p></o:p>
3. Profiles of the DGGE banding
patterns suggested that ecophysiological characteristics differ among members
of the genus Methylobacter;
this indicates the importance of investigating the MOB assemblage at the
species level or lower. Planktonic MOB seemed to be abundant around oxycline.<o:p></o:p>
<o:p> </o:p>
また、博士研究員・東岡由里子さんの研究もJournal
of Applied Microbiology誌に受理されました。詳細は、オンラインで公開された時にご紹介いたします。<o:p></o:p>
<o:p> </o:p>
著者：Yuriko Higashioka,
Hisaya Kojima and Manabu Fukui.<o:p></o:p>
題名：Temperature-dependent
differences in community structure of bacteria involved in degradation of
petroleum hydrocarbons under sulfate-reducing conditions.<o:p></o:p>
<o:p> </o:p>
