週末札幌散歩：さっぽろオータムフェスト2017

秋晴れ。夕方、大通公園で開催されている「さっぽろオータムフェスト2017」へ。本日が最終日と言うこともあり、大賑わい。







６丁目会場にて、「スープカレー vs人気ルウカレー対決」開催。『おにぎりあたためますか』で紹介された「E-itou Curry（エイトカリー）」が出店中と言うことで、「オータム10回記念限定　北海道産焦がしチーズ入りハンバーグカレー」を頂く。すこぶる美味！







お次ぎは、「無水パキスタン風カレー」。



そして、熱々の新作カレーパン。



『おにぎりあたためますか』で大泉洋が熱く語っていたように、複数人で違うカレーを注文し、シェアして、少しずつ食べ比べするのが良いかと思います。

カレーで身体も温まったので、さあ、帰宅しよう。

札幌ドームにて野球観戦

内地より来客あり。せっかくの機会なので、札幌ドームへ。この日は、日ハム−楽天戦。







３対３で迎えた９回裏。日ハムの西川選手によるサヨナラ打。日ハムの勝利で札幌ドームは盛り上がり。



気軽に野球観戦できるのも札幌暮らしの利点。来客大歓迎です。

オコタンペ湖から新種の好酸性微生物

当研究室で主たる研究フィールドであるオコタンペ湖。微生物によるメタン酸化や硫黄酸化を中心に研究を行ってきましたが、好酸性微生物の新種を発見いたしました。

従属栄養性なのですが、生育pHが3.0から6.2です（至適pHは4.5）。今年３月に修士課程を修了した岡本怜さんの研究成果です。彼女の努力が実りました。

Rei Okamoto, Hisaya Kojima and Manabu Fukui.
Acidocella aquatica sp. nov., a novel acidophilic heterotrophic bacterium isolated from a freshwater lake. International Journal of Systematics and Evolutionary Microbiology. In press.



(注意)オコタンペ湖は国立公園内にあるため、調査や試料の採取には環境省等からの許可が必要です。当研究室では、許可を得て研究活動を行っています。

研究論文update170916

【2017年以降】

*Rei Okamoto, Hisaya Kojima and Manabu Fukui. Acidocella aquatica sp. nov., a novel acidophilic heterotrophic bacterium isolated from a freshwater lake. International Journal of Systematics and Evolutionary Microbiology. In press.

* Masayuki Itoh, Hisaya Kojima, Pei-Chi Ho, Chun-Wei Chang, Tzong-Yueh Chen, Silver Sung-Yun Hsiao, Yuki Kobayashi, Megumi Fujibayashi, Shuh-Ji Kao, Chih-hao Hsieh, Manabu Fukui, Noboru Okuda, Takeshi Miki and Fuh-Kwo Shiah. Integrating isotopic, microbial, and modeling approaches to understand methane dynamics in a frequently disturbed deep reservoir in Taiwan. Ecological Research. In press.

*. Mia Terashima, Kazuhiro Umezawa, Shoichi Mori, Hisaya Kojima and Manabu Fukui. Microbial community structure analysis of colored snow from alpine snowfield in northern Japan reveals the prevalence of Betaproteobacteria with snow algae. Frontiers in Microbiology 8: 1481. doi:10.3389/fmicb.2017.01481

*. Miho Watanabe , Hisaya Kojima and Manabu Fukui. Complete genome sequence of Marinifilaceae bacterium strain SPP2, isolated from Antarctic marine sediment. Marine Genomics: in press

*. Hisaya Kojima, Miho Watanabe and Manabu Fukui. Sulfurivermis fontis gen. nov., sp. nov., a novel sulfur-oxidizing autotroph, and proposal of Thioprofundaceae fam. nov. International Journal of Systematic and Evolutionary Microbiology 67: 3458-3461. 2017.

*. Tomohiro Watanabe, Aya Miura, Tomoya Iwata, Hisaya Kojima and Manabu Fukui. Dominance of Sulfuritalea species in nitrate-depleted water of a stratified freshwater lake and arsenate respiration ability within the genus. Environmental Microbiology Reports 9: 522-527. 2017.

*. Miho Watanabe, Hisaya Kojima and Manabu Fukui. Draft genome sequence of Desulfoplanes formicivorans Pf12BT, a sulfate-reducing bacterium of the family Desulfomicrobiaceae. Standards in Genomic Sciences 2: 34. 2017.DOI:

*. Miho Watanabe, Yuriko Higashioka, Hisaya Kojima and Manabu Fukui. Desulfosarcina widdelii sp. nov. and Desulfosarcina alkanivorans sp. nov., hydrocarbon-degrading sulfate-reducing bacteria isolated from marine sediment and emended description of the genus Desulfosarcina. International Journal of Systematic and Evolutionary Microbiology 67: 2994-2997. 2017.DOI:

*. Miho Watanabe, Hisaya Kojima and Manabu Fukui. Desulfocucumis palustris gen. nov., sp. nov., a mesophilic sulfate reducer belonging to the Desulfotomaculum subcluster Ig. International Journal of Systematic and Evolutionary Microbiology 67: 2679-2682. 2017. DOI:

*. Hisaya Kojima, Miho Watanabe, and Manabu Fukui. Sulfuritortus calidifontis gen. nov., sp. nov., a novel sulfur oxidizer isolated from a hot spring microbial mat. International Journal of Systematic and Evolutionary Microbiology 67:1355-1358. 2017. DOI: 10.1099/ijsem.0.001813


【2016年】

*. Tomohiro Watanabe, Hisaya Kojima and Manabu Fukui.Identity of major sulfur cycle prokaryotes in freshwater lake ecosystems revealed by a comprenensive phylogenetic study of the dissimilatory adenylylsulfate reductase. Scientific Reports 6: 36262. 2016.

*. Kazuhiro Umezawa, Tomohiro Watanabe, Aya Miura, Hisaya Kojima and Manabu Fukui. The complete genome sequences of sulfur-oxidizing gammaproteobacteria Sulfurifustis variabilis skN76T and Sulfuricaulis limicola HA5T. Standards in Genomic Sciences 11: 71. 2016.

*. Hisaya Kojima, Miho Watanabe, Riho Tokizawa, Arisa Shinohara and Manabu Fukui. Hymenobacter nivis sp. nov., isolated from red snow in Antarctica. International Journal of Systematic and Evolutionary Microbiology 66: 4821-4825. 2016. DOI: 10.1099/ijsem.0.001435

*. Yuki Kobayashi, Hisaya Kojima, Masayuki Itoh, Noboru Okuda, Manabu Fukui and Fuh-Kwo Shiah. Abundance of planktonic methane-oxidizing bacteria in a subtropical reservoir. Plankton and Benthos Research 11: 144-146.2016. DOI: 10.3800/pbr.11.144

*. Hisaya Kojima and Manabu Fukui. Sulfuriflexus mobilis gen. nov., sp. nov., a sulfur-oxidizing bacterium isolated from a brackish lake sediment. International Journal of Systematic and Evolutionary Microbiology 66: 3515-3518.2016. doi: 10.1099/ijsem.0.001227

*. Rachel Rudd, Jonathan Tyler, John Tibby, Yusuke Yokoyama, Ines Tavernier, Elie Verleyen, Manabu Fukui and Yoshinori Takano. A diatom inferred of lake hydrological variability during the last 900 years in Luetzow-Holm Bay, East Antarctica. Journal of Quaternary Science 31: 114-125. 2016. DOI: 10.1002/jqs.2845


*. Tomohiro Watanabe, Hisaya Kojima and Manabu Fukui. Sulfuriferula thiophila sp. nov., a chemolithoautotrophic sulfur-oxidizing bacterium, and correction of the name Sulfuriferula plumbophilus Watanabe, Kojima and Fukui 2015 to Sulfuriferula plumbophila corrig.　International Journal of Systematic and Evolutionary Microbiology 66: 2041-2045.2016. Doi: 10.1099/ijsem.0.000988.

*. Masanori Fujii, Toshihide Hirao, Hisaya Kojima and Manabu Fukui. Planktonic bacterial communities in mountain lake ecosystems. In: Structure and Function of Mountain Ecosystems in Japan. 145-169.Springer. 2016.

*. Tomoya Iwata, Naoki Mochizuki, Takao Suzuki, Ayato Kohzu, Hisaya Kojima, Manabu Fukui and Jotaro Urabe. Roles of terrestrial carbon subsidies to aquatic community metabolism in mountain lake ecosystems. In: Structure and Function of Mountain Ecosystems in Japan. 115-144. Springer. 2016.

*. Tomohiro Watanabe, Aya Miura, Arisa Shinohara, Hisaya Kojima and Manabu Fukui. Thermithiobacillus plumbiphilus sp. nov., a sulfur-oxidizing bacterium isolated from lead sulfide.　International Journal of Sysytematic and Evolutionary Microbiology 66: 1986-1989.2016. Doi: 10.1099/ijsem.0.000972.

*. Hisaya Kojima, Kazuhiro Umezawa and Manabu Fukui. Caldimicrobium thiodismutans sp. nov., a sulfur-disproportionating bacterium isolated from a hot spring, and emended description of the genus Caldimicrobium. International Journal of Systematics and Evolutionary Microbiology 66: 1828-1831.2016. Doi: 10.1099/ijsem.0.000947.

*. Miho Watanabe, Hisaya Kojima and Manabu Fukui. Complete genome sequence and cell structure of Limnochorda pilosa, a Gram negative spore former whithin the phylum Firmicutes. International Journal of Systematic and Evolutionary Microbiology 66: 1330-1339. 2016. Doi:10.1099/ijsem.0.000881.

*. Hisaya Kojima, Tomohiro Watanabe and Manabu Fukui. Sulfuricaulis limicola gen. nov., sp. nov., a novel sulfur oxidizer from a lake. International Journal of Systematic and Evolutionary Microbiology 66: 266-270. 2016. Doi: 10.1099/ijsem.0.000709.

*. Tomohiro Watanabe, Hisaya Kojima, Arisa Shinohara and Manabu Fukui. Sulfurirhabdus autotrophica gen. nov., sp. nov., isolated from a freshwater lake.　International Journal of Systematic and Evolutionary Microbiology 66: 113-117.2016. Doi: 10.1099/ijsem.0.000679.


【2015年】

*. Yuriko Higashioka, Hisaya Kojima, Tomohiro Watanabe and Manabu Fukui. Draft genome sequence of Desulfatitalea tepidiphila S28bFT. Genome Announcements 3: e01326-15. 2015.

*. Tomohiro Watanabe, Hisaya Kojima and Manabu Fukui. Draft genome sequence of Mizugakiibacter sediminis skMP5T. Genome Announcements 3: e01185-15. 2015.

*. Ikumi Oyabu, Yoshinori Iizuka, Hubertus Fischer, Simon Schüpbach, Gideon Gfeller, Anders Svensson, Manabu Fukui, Jørgen Peder Steffensen, and Margareta Hansson. Chemical compositions of solid particles present in the Greenland NEEM ice core over the last 110,000 years. Journal of Geophysical Research-Atmospheres 120: 9789-9813.2015. DOI: 10.1002/2015JD023290

*. Hisaya Kojima, Arisa Shinohara and Manabu Fukui. Sulfurifustis variabilis gen. nov., sp. nov., a novel sulfur oxidizer isolated from a lake, and proposal of Acidiferrobacteraceae fam. nov. and Acidiferrobacterales ord. nov. International Journal of Systematic and Evolutionary Microbiology 65: 3709-3713.2015. Doi:10.1099/ijsem.0.000479

*. Masayuki Itoh, Yuki Kobayashi, Tzong-Yueh Chen, Takeshi Tokida, Manabu Fukui, Hisaya Kojima, Takeshi Miki, Ichiro Tayasu, Fuh-Kwo Shiah and Noboru Okuda. Effect of inter-annual variation in winter vertical mixing on CH4 dynamics in a subtropical reservoir. Journal of Geophysics Research-Biogeoscience 120: 1246-1261. 2015.

*. Masanori Fujii, Toshihide Hirao, Hisaya Kojima and Manabu Fukui. Inter-taxonomic differences in bacterioplankton community assembly in oligotrophic mountain lakes of east Japan. Aquatic Microbial Ecology 75: 251-257. 2015.

*. Tomohiro Watanabe, Hisaya Kojima and Manabu Fukui. Draft genome sequence of a sulfur-oxidizing autotroph, Sulfuricella sp. strain T08, isolated from a freshwater lake. Genome Announcements 3: e00498-15. 2015.

*. Miho Watanabe, Hisaya Kojima, Manabu Fukui. Limnochorda pilosa gen. nov., sp. nov., a moderately thermophilic, facultative anaerobic pleomorphic bacterium and proposal of Limnochordaceae fam. nov., Limnochordales ord. nov. and Limnochordia classis nov. in the phylum Firmicutes. International Journal of Systematic and Evolutionary Microbiology 65: 2378-2394.2015. Doi:10.1099/ijs.0.000270

*. Miho Watanabe, Hisaya Kojima and Manabu Fukui. Desulfoplanes formicivorans gen. nov., sp. nov., a novel sulfate-reducing bacterium isolated from a brackish meromictic lake, and emended description of the family Desulfomicrobiaceae. International Journal of Systematic and Evolutionary Microbiology 65: 1902-1907.2015.

*. Tomohiro Watanabe, Hisaya Kojima and Manabu Fukui. Sulfuriferula multivorans gen. nov., sp. nov., isolated from a freshwater lake, reclassification of 'Thiobacillus plumbophilus' as Sulfuriferula plumbophilus sp. nov., and description of Sulfuricellaceae fam. nov. and Sulfuricellales ord. nov.　International Journal of Systematic and Evolutionary Microbiology 65: 1504-1508.2015.

*. Yoshinori Takano, Hisaya Kojima, Eriko Takeda, Yusuke Yokoyama and Manabu Fukui. Biogeochemistry and limnology in Antarctic subglacial weathering: molecular evidence of the linkage between subglacial silica input and primary producers in a perennially ice-covered lake. Progress in Earth and Planetary Science 2: 8 (pp15). 2015.

*. Hisaya Kojima, Yoshitoshi Ogura, Nozomi Yamamoto, Tomoaki Togashi, Hiroshi Mori, Tomohiro Watanabe, Fumiko Nemoto, Ken Kurokawa, Tetsuya Hayashi and Manabu Fukui. Ecophysiology of Thioploca ingrica as revealed by the complete genome sequence supplemented with proteomic evidence. The ISME Journal 9: 1166-1176.2015.

【2014年】
*. Hisaya Kojima, Riho Tokizawa and Manabu Fukui. Mizugakiibacter sediminis gen. nov., sp. nov., isolated from a freshwater lake. International Journal of Systematic and Evolutionary Microbiology 64: 3983-3987. 2014.

*. Hisaya Kojima, Riho Tokizawa, Kouhei Kogure, Yuki Kobayashi, Masayuki Itoh, Fuh-Kuw Shiah, Noboru Okuda and Manabu Fukui. Community structure of planktonic methane-oxidizing bacteria in a subtropical reservoir characterized by dominance of phylotype closely related to nitrite reducer. Scientific Reports 4: 5728. DOI: 10.1038/srep05728.

*. Tomohiro Watanabe, Hisaya Kojima, and Manabu Fukui. Complete genomes of freshwater sulfur oxidizers Sulfuricella denitrificans skB26 and Sulfuritalea hydrogenivorans sk43H: Genetic insights into the sulfur oxidation pathway of betaproteobacteria. Systematic and Applied Microbiology 37: 387-395.2014.

*. Miho Watanabe, Hisaya Kojima and Manabu Fukui. Proposal of Effusibacillus lacus gen. nov., sp. nov. and reclassification of Alicyclobacillus pohliae as Effusibacillus pohliae comb. nov. and Alicyclobacillus consociatus as Effusibacillus consociatus comb. nov.. International Journal of Systematic and Evolutionary Microbiology 64: 2770-2774.2014.

*. Kyoko Kubo, Hisaya Kojima, and Manabu Fukui. Vertical distribution of major sulfate-reducing bacteria in a eutrophic shallow meromictic lake. Systematic and Applied Microbiology 37: 510-519. 2014.

*. Hisaya Kojima, Johanna Moll, Joerg Kahnt, Manabu Fukui and Seigo Shima. A reversed genetic approach reveals the coenzyme specificity and the catalytic properties of three enzymes putatively involved in anaerobic oxidation of methane with sulfate. Environmental Microbiology 16: 3431-3442. 2014.

*. Hisaya Kojima, Tomohiro Watanabe, Tomoya Iwata and Manabu Fukui. Identification of major planktonic sulfur oxidizers in stratified freshwater lake. PLoS ONE : e93877. 2014.

*. Hisaya Kojima and Manabu Fukui. Sulfurisoma sediminicola gen. nov., sp. nov., a novel facultative autotroph isolated from a freshwater lake. International Journal of Systematic and Evolutionary Microbiology 64: 1587-1592. 2014.

【2013年】

*. Tomohiro Watanabe, Hisaya Kojima, Yoshinori Takano and Manabu Fukui. Diversity of sulfur-cycle prokaryotes in freshwater lake sediments investigated using aprA as functional marker gene. Systematic and Applied Microbiology 36: 436-443.2013.

*. Yoshinori Iizuka, Barbara Delmonte, Ikumi Oyabu, Torbjoern Karlin, Valter Maggi, Amuel Albani, Manabu Fukui, Takeo Hondoh and Margareta Hansson. Sulphate and chloride aerosols during Holocene and last glacial periods preserved in the Talos Dome Ice Core, a peripheral region of Antarctica. Tellus B 65: 20197.2013.

*. Miho Watanabe, Hisaya Kojima and Manabu Fukui. Desulfotomaculum intricatum sp. nov., a sulfate-reducer isolated from freshwater lake sediment. International Journal of Systematic and Evolutionary Microbiology 63: 3574-3578. 2013.

*.Takato Matsui, Hisaya Kojima, and Manabu Fukui. Effects of temperature on successive anaerobic decomposition of high-molecular organic matter under sulfate-reducing conditions. Estuarine, Coastal and Shelf Science 119: 139-144. 2013.

*. Akiko Kamono, Marianne Meyer, Thomas Cavalier-Smith,　Manabu Fukui and Anne Maria Fiore-Donno. Exploring slime mould diversity in high altitude forests and grasslands by environmental RNA analysis. FEMS Microbiology Ecology 84: 98-108. 2013.

*. Yuriko Higashioka, Hisaya Kojima, Miho Watanabe and Manabu Fukui. Desulfatitalea tepidiphila gen. nov., sp. nov., a novel sulfate-reducing bacterium isolated from tidal flat sediment. International Journal of Systematic and Evolutionary Microbiology 63: 761-765. 2013.

【2012年】

*. Yoshinori Takano, Jonathan James Tyler, Hisaya Kojima, Yusuke Yokoyama, Yukiko Tanabe, Takaharu Sato, Nanako O. Ogawa, Naohiko Ohkouchi, and Manabu Fukui. Holocene lake developments and glacial-isostatic uplift at Lake Skallen and Lake Oyako, Luetzow-Holm Bay, east Antarctica: based on biogeochemical facies and molecular signatures. Applied Geochemistry 27: 2546-2559. 2012.

*. Tomohiro Watanabe, Hisaya Kojima, and Manabu Fukui. Draft genome sequence of a psychrotolerant sulfur-oxidizing bacterium Sulfuricella denitrificans skB26 and proteomic insights into cold adaptation. Applied and Environmental Microbiology 78: 6545-6549. 2012.

*. Fumiko Nemoto, Hisaya Kojima, Akifumi Ohtaka and Manabu Fukui. Filamentous sulfur-oxidizing bacteria of the genus Thioploca from Lake Tonle Sap in Cambodia. Aquatic Microbial Ecology 66: 295-300. 2012.

*. Anne Maria Fiore-Donno, Akiko Kamono, Marianne Meyer, Martin Schnittler, Manabu Fukui and Thomas Cavalier-Smith. 18S rDNA phylogeny of Lanmproderma and allied genera (Stemonitales, Myxomycetes, Amoebozoa). PLoS ONE 7: e35359. 2012.

*. Hisaya Kojima, Masazumi Tsutsumi, Kanako Ishikawa, Tomoya Iwata, Marc Mussmann and Manabu Fukui. Distribution of putative denitrifying methane oxidizing bacteria of a freshwater lake. Systematic and Applied Microbiology 35: 233-238. 2012.

*. Yuriko Higashioka, Hisaya Kojima, and Manabu Fukui. Isolation and characterization of novel sulfate-reducing bacterium capable anaerobic degradation of p-xylene. Microbes and Environments 27: 273-277. 2012.

*. Masanaori Fujii, Hisaya Kojima, Tomoya Iwata, Jotaro Urabe and Manabu Fukui. Dissolved organic carbon as major environmental factor affecting bacterioplankton communities in mountain lakes of eastern Japan. Microbial Ecology 63: 496-508. 2012.

*. Masazumi Tsutsumi, Hisaya Kojima, and Manabu Fukui. Vertical profiles of abundance and potential activity of methane-oxidizing bacteria in sediment of Lake Biwa, Japan. Microbes and Environments 27: 67-71. 2012.

【2011年】

*. Fumiko Nemoto, Hisaya Kojima, and Manabu Fukui. Diversity of freshwater Thioploca species and their specific association with filamentous bacteria of the phylum Chloroflexi. Microbial Ecology 62：753-764.2011.

*. Hisaya Kojima, and Manabu Fukui. Sulfuritalea hydrogenivorans gen. nov., sp. nov., a novel facultative autotroph isolated from a freshwater lake. International Journal of Systematic and Evolutionary Microbiology 61: 1651-1655. 2011.

*. Masazumi Tsutsumi, Tomoya Iwata, Hisaya Kojima, and Manabu Fukui. Spatiotemporal variations in an assemblage of closely related planktonic aerobic methanotrophs. Freshwater Biology 56: 342-351. 2011.

*. Yuriko Higashioka, Hisaya Kojima and Manabu Fukui. Temperature-dependent differences in community structure of bacteria involved in degradation of petroleum hydrocarbons under sulfate-reducing conditions. Journal of Applied and Microbiology　110: 314-322. 2011.

*. Kyoko Kubo, Katrin Knittel, Rudolf Amann, Manabu Fukui and Katsumi Matsuura. Sulfur-metabolizing bacteria populations in microbial mats of the Nakabusa hot spring, Japan. Systematic and Applied Microbiology 34: 293-302, 2011.

【2010年】

*. Hisaya Kojima, and Manabu Fukui. Sulfuricella denitrificans gen. nov., sp. nov., a novel sulfur-oxidizing autotroph isolated from a freshwater lake. International Journal of Systematic and Evolutionary Microbiology 60:2862-2866. 2010.

*. Keiichi Tabuchi, Hisaya Kojima, and Manabu Fukui. Seasonal changes in organic matter mineralization in a sublittoral sediment and temperature-driven decoupling of key processes. Microbial Ecology 60: 551-560. 2010.

*. Masanaori Fujii, Yoshinori Takano, Hisaya Kojima, Tamotsu Hoshino, Ryoichi Tanaka and Manabu Fukui. Microbial community structure, pigment composition, and nitrogen source of red snow in Antarctica. Microbial Ecology 59: 466-4775.2010.

*. Anne Fiore-Donno, Akiko Kamono, Ema Chao, Manabu Fukui and Thomas Cavalier-Smith. Invalidation of hyperamoeba by transferring its species to other genera of Myxogastria. Journal of Eukaryotic Biology 52: 189-196. 2010.

【2009年】

*. Akiko Kamono, Hisaya Kojima, Jun Matsumoto, Kimitaka Kawamura and Manabu Fukui. Airborne Myxomycete spore: Detection using molecular techniques. Naturwissenschaften　96: 147-151.2009.

*. Masazumi Tsutsumi, Hisaya Kojima, Shigeru Uemura, Kiyomi Ono, Akihiro Sumida, Toshihiko Hara and Manabu Fukui. Structure and activity of soil-inhabiting methanotrophic communities in northern forest of Japan. Soil Biology and Biochemistry　 41: 403-408.2009.

*. Yuriko Higashioka, Hisaya Kojima, Tatsunori Nakagawa, Shinya Sato and Manabu Fukui. A novel n-alkane-degrading bacterium as a minor member of p-xylene-degrading sulfate-reducing consortium. Biodegradation　20: 383-390.2009.　

*. Yuriko Higashioka, Hisaya Kojima, Shinya Sato, Manabu Fukui. Microbial community analysis at crude oil-contaminated soils targeting the 16S ribosomal RNA, xylM, C23O, and bcr genes. Journal of Applied Microbiology　107: 126-135. 2009.

*. Hisaya Kojima, Tomoya Iwata, and Manabu Fukui. DNA-based analysis of planktonic methanotrophs in a stratified lake. Freshwater Biology　54: 1501-1509.2009.

*. Akiko Kamono, Jun Matsumoto, Hisaya Kojima, and Manabu Fukui. Characterization of myxomycete communities in soil by reverse transcription polymerase chain reaction (RT-PCR)-based method. Soil Biology and Biochemistry 41: 1324-1330. 2009.

*. Hisaya Kojima, Haruo Fukuhara and Manabu Fukui. Community structure of microorganisms asssociated with reddish-brown iron-rich snow. Systematic and Applied Microbiology 32: 429-437. 2009.

海洋研究の魅力

ブレーメン大学Wilhelm Hagen教授。北海道大学南極学カリキュラムでは多大な貢献をして頂いている先生ですが、この度一般向けの本を出版されたと言うことで、献本して頂きました。きっと、１０月開講の「南極学特別講義II」で、Hagen教授から紹介されることでしょう。

Hempel, Bischof, Hagen編著
Faszination Meereforschung
Springer, 2017





アルフレッドウェゲナー極地海洋研究所のKarin Lochte所長、また、Antje Boetius次期所長の書き物も含まれています。写真や図も素晴らしく、一般読者を魅了しています。

唯一の難点は、ドイツ語で書かれていること。日本語訳が出版されることを切望します。

週末札幌散歩：おにぎりあたためますか

ローカルテレビ局HTB（北海道テレビ放送）の番組『おにぎりあたためますか』。大泉洋が出演する深夜バラエティ番組なのですが、ついつい視てしまう面白さがあります。

2004年夏、東京から札幌に赴任した当時のこと。コンビニでおにぎりを買うと、必ず店員さんに「おにぎりあたためますか」と聞かれ、戸惑ったことを思い出します。今では、当たり前のことのように思えます。

さて、高校時代の友人A君が新潟から札幌にやってきました。39年ぶりの再会です。ホテルのロビーで待ち合わせをして、早速、地下鉄南北線に乗って「中の島」へ。地下鉄駅から寒地土木研究所方面へ徒歩７分。目的地は、エイトカリー（E-itou curry）。ここは高校の同級生B君が営むカレー屋。



私はいつもの「手仕込みハンバーグカレー」（辛さ３番をチョイス）、A君は「ふわとろオムレツのカレー」を美味しくいただく。週末なので、サッポロクラシック（ビール）も。超ローカルかつ昔話で盛り上がりました。

このエイトカレーですが、『おにぎりあたためますか』の取材が入ったとのこと。９月１２日放映予定です。

お店の壁には、大泉洋のサインも掲げられていました。ローカルな番組ですが、CSのテレ朝chでも視聴できるらしいです。



故郷から遠く離れた札幌まで足を運んでくれた同級生。すこぶる嬉しいです！



（店主のB君と３人で）

プレスリリース：北海道最高峰の旭岳に染まる彩雪の謎を探る〜

本日、研究成果のプレスリリースを行いました。
詳細はここからご確認いただけます。

９月４日、全学停電のため、９月１日１６時より９月４日１２時まで北海道大学のホームページが停止いたしますので、上記からプレスリリース原稿をダウンロードできなくなります。ご容赦ください。

Mia Terashima, Kazuhiro Umezawa, Shoichi Mori, Hisaya Kojima and Manabu Fukui. Microbial community structure analysis of colored snow from alpine snowfield in northern Japan reveals the prevalence of Betaproteobacteria with snow algae. Frontiers in Microbiology. doi:10.3389/fmicb.2017.01481

研究論文update170901

【2017年以降】

*. Mia Terashima, Kazuhiro Umezawa, Shoichi Mori, Hisaya Kojima and Manabu Fukui. Microbial community structure analysis of colored snow from alpine snowfield in northern Japan reveals the prevalence of Betaproteobacteria with snow algae. Frontiers in Microbiology. doi:10.3389/fmicb.2017.01481

*. Miho Watanabe , Hisaya Kojima and Manabu Fukui. Complete genome sequence of Marinifilacea bacterium strain SPP2, isolated from Antarctic marine sediment. Marine Genomics: in press

*. Hisaya Kojima, Miho Watanabe and Manabu Fukui. Sulfurivermis fontis gen. nov., sp. nov., a novel sulfur-oxidizing autotroph, and proposal of Thioprofundaceae fam. nov. International Journal of Systematic and Evolutionary Microbiology . in press

*. Tomohiro Watanabe, Aya Miura, Tomoya Iwata, Hisaya Kojima and Manabu Fukui. Dominance of Sulfuritalea species in nitrate-depleted water of a stratified freshwater lake and arsenate respiration ability within the genus. Environmental Microbiology Reports : . In press.

*. Miho Watanabe, Hisaya Kojima and Manabu Fukui. Draft genome sequence of Desulfoplanes formicivorans Pf12BT, a sulfate-reducing bacterium of the family Desulfomicrobiaceae. Standards in Genomic Sciences: in press. DOI:

*. Miho Watanabe, Hisaya Kojima and Manabu Fukui. Desulfosarcina widdelii sp. nov. and Desulfosarcina alkanivorans sp. nov., hydrocarbon-degrading sulfate-reducing bacteria isolated from marine sediment and emended description of the genus Desulfosarcina. International Journal of Systematic and Evolutionary Microbiology : in press. DOI:

*. Miho Watanabe, Hisaya Kojima and Manabu Fukui. Desulfocucumis palustris gen. nov., sp. nov., a mesophilic sulfate reducer belonging to the Desulfotomaculum subcluster Ig. International Journal of Systematic and Evolutionary Microbiology : in press. DOI:

*. Hisaya Kojima, Miho Watanabe, and Manabu Fukui. Sulfuritortus calidifontis gen. nov., sp. nov., a novel sulfur oxidizer isolated from a hot spring microbial mat. International Journal of Systematic and Evolutionary Microbiology : in press. DOI: 10.1099/ijsem.0.001813


【2016年】

*. Tomohiro Watanabe, Hisaya Kojima and Manabu Fukui.Identity of major sulfur cycle prokaryotes in freshwater lake ecosystems revealed by a comprenensive phylogenetic study of the dissimilatory adenylylsulfate reductase. Scientific Reports 6: 36262. 2016.

*. Kazuhiro Umezawa, Tomohiro Watanabe, Aya Miura, Hisaya Kojima and Manabu Fukui. The complete genome sequences of sulfur-oxidizing gammaproteobacteria Sulfurifustis variabilis skN76T and Sulfuricaulis limicola HA5T. Standards in Genomic Sciences 11: 71. 2016.

*. Hisaya Kojima, Miho Watanabe, Riho Tokizawa, Arisa Shinohara and Manabu Fukui. Hymenobacter nivis sp. nov., isolated from red snow in Antarctica. International Journal of Systematic and Evolutionary Microbiology 66: 4821-4825. 2016. DOI: 10.1099/ijsem.0.001435

*. Yuki Kobayashi, Hisaya Kojima, Masayuki Itoh, Noboru Okuda, Manabu Fukui and Fuh-Kwo Shiah. Abundance of planktonic methane-oxidizing bacteria in a subtropical reservoir. Plankton and Benthos Research 11: 144-146.2016. DOI: 10.3800/pbr.11.144

*. Hisaya Kojima and Manabu Fukui. Sulfuriflexus mobilis gen. nov., sp. nov., a sulfur-oxidizing bacterium isolated from a brackish lake sediment. International Journal of Systematic and Evolutionary Microbiology 66: 3515-3518.2016. doi: 10.1099/ijsem.0.001227

*. Rachel Rudd, Jonathan Tyler, John Tibby, Yusuke Yokoyama, Ines Tavernier, Elie Verleyen, Manabu Fukui and Yoshinori Takano. A diatom inferred of lake hydrological variability during the last 900 years in Luetzow-Holm Bay, East Antarctica. Journal of Quaternary Science 31: 114-125. 2016. DOI: 10.1002/jqs.2845


*. Tomohiro Watanabe, Hisaya Kojima and Manabu Fukui. Sulfuriferula thiophila sp. nov., a chemolithoautotrophic sulfur-oxidizing bacterium, and correction of the name Sulfuriferula plumbophilus Watanabe, Kojima and Fukui 2015 to Sulfuriferula plumbophila corrig.　International Journal of Systematic and Evolutionary Microbiology 66: 2041-2045.2016. Doi: 10.1099/ijsem.0.000988.

*. Masanori Fujii, Toshihide Hirao, Hisaya Kojima and Manabu Fukui. Planktonic bacterial communities in mountain lake ecosystems. In: Structure and Function of Mountain Ecosystems in Japan. 145-169.Springer. 2016.

*. Tomoya Iwata, Naoki Mochizuki, Takao Suzuki, Ayato Kohzu, Hisaya Kojima, Manabu Fukui and Jotaro Urabe. Roles of terrestrial carbon subsidies to aquatic community metabolism in mountain lake ecosystems. In: Structure and Function of Mountain Ecosystems in Japan. 115-144. Springer. 2016.

*. Tomohiro Watanabe, Aya Miura, Arisa Shinohara, Hisaya Kojima and Manabu Fukui. Thermithiobacillus plumbiphilus sp. nov., a sulfur-oxidizing bacterium isolated from lead sulfide.　International Journal of Sysytematic and Evolutionary Microbiology 66: 1986-1989.2016. Doi: 10.1099/ijsem.0.000972.

*. Hisaya Kojima, Kazuhiro Umezawa and Manabu Fukui. Caldimicrobium thiodismutans sp. nov., a sulfur-disproportionating bacterium isolated from a hot spring, and emended description of the genus Caldimicrobium. International Journal of Systematics and Evolutionary Microbiology 66: 1828-1831.2016. Doi: 10.1099/ijsem.0.000947.

*. Miho Watanabe, Hisaya Kojima and Manabu Fukui. Complete genome sequence and cell structure of Limnochorda pilosa, a Gram negative spore former whithin the phylum Firmicutes. International Journal of Systematic and Evolutionary Microbiology 66: 1330-1339. 2016. Doi:10.1099/ijsem.0.000881.

*. Hisaya Kojima, Tomohiro Watanabe and Manabu Fukui. Sulfuricaulis limicola gen. nov., sp. nov., a novel sulfur oxidizer from a lake. International Journal of Systematic and Evolutionary Microbiology 66: 266-270. 2016. Doi: 10.1099/ijsem.0.000709.

*. Tomohiro Watanabe, Hisaya Kojima, Arisa Shinohara and Manabu Fukui. Sulfurirhabdus autotrophica gen. nov., sp. nov., isolated from a freshwater lake.　International Journal of Systematic and Evolutionary Microbiology 66: 113-117.2016. Doi: 10.1099/ijsem.0.000679.


【2015年】

*. Yuriko Higashioka, Hisaya Kojima, Tomohiro Watanabe and Manabu Fukui. Draft genome sequence of Desulfatitalea tepidiphila S28bFT. Genome Announcements 3: e01326-15. 2015.

*. Tomohiro Watanabe, Hisaya Kojima and Manabu Fukui. Draft genome sequence of Mizugakiibacter sediminis skMP5T. Genome Announcements 3: e01185-15. 2015.

*. Ikumi Oyabu, Yoshinori Iizuka, Hubertus Fischer, Simon Schüpbach, Gideon Gfeller, Anders Svensson, Manabu Fukui, Jørgen Peder Steffensen, and Margareta Hansson. Chemical compositions of solid particles present in the Greenland NEEM ice core over the last 110,000 years. Journal of Geophysical Research-Atmospheres 120: 9789-9813.2015. DOI: 10.1002/2015JD023290

*. Hisaya Kojima, Arisa Shinohara and Manabu Fukui. Sulfurifustis variabilis gen. nov., sp. nov., a novel sulfur oxidizer isolated from a lake, and proposal of Acidiferrobacteraceae fam. nov. and Acidiferrobacterales ord. nov. International Journal of Systematic and Evolutionary Microbiology 65: 3709-3713.2015. Doi:10.1099/ijsem.0.000479

*. Masayuki Itoh, Yuki Kobayashi, Tzong-Yueh Chen, Takeshi Tokida, Manabu Fukui, Hisaya Kojima, Takeshi Miki, Ichiro Tayasu, Fuh-Kwo Shiah and Noboru Okuda. Effect of inter-annual variation in winter vertical mixing on CH4 dynamics in a subtropical reservoir. Journal of Geophysics Research-Biogeoscience 120: 1246-1261. 2015.

*. Masanori Fujii, Toshihide Hirao, Hisaya Kojima and Manabu Fukui. Inter-taxonomic differences in bacterioplankton community assembly in oligotrophic mountain lakes of east Japan. Aquatic Microbial Ecology 75: 251-257. 2015.

*. Tomohiro Watanabe, Hisaya Kojima and Manabu Fukui. Draft genome sequence of a sulfur-oxidizing autotroph, Sulfuricella sp. strain T08, isolated from a freshwater lake. Genome Announcements 3: e00498-15. 2015.

*. Miho Watanabe, Hisaya Kojima, Manabu Fukui. Limnochorda pilosa gen. nov., sp. nov., a moderately thermophilic, facultative anaerobic pleomorphic bacterium and proposal of Limnochordaceae fam. nov., Limnochordales ord. nov. and Limnochordia classis nov. in the phylum Firmicutes. International Journal of Systematic and Evolutionary Microbiology 65: 2378-2394.2015. Doi:10.1099/ijs.0.000270

*. Miho Watanabe, Hisaya Kojima and Manabu Fukui. Desulfoplanes formicivorans gen. nov., sp. nov., a novel sulfate-reducing bacterium isolated from a brackish meromictic lake, and emended description of the family Desulfomicrobiaceae. International Journal of Systematic and Evolutionary Microbiology 65: 1902-1907.2015.

*. Tomohiro Watanabe, Hisaya Kojima and Manabu Fukui. Sulfuriferula multivorans gen. nov., sp. nov., isolated from a freshwater lake, reclassification of 'Thiobacillus plumbophilus' as Sulfuriferula plumbophilus sp. nov., and description of Sulfuricellaceae fam. nov. and Sulfuricellales ord. nov.　International Journal of Systematic and Evolutionary Microbiology 65: 1504-1508.2015.

*. Yoshinori Takano, Hisaya Kojima, Eriko Takeda, Yusuke Yokoyama and Manabu Fukui. Biogeochemistry and limnology in Antarctic subglacial weathering: molecular evidence of the linkage between subglacial silica input and primary producers in a perennially ice-covered lake. Progress in Earth and Planetary Science 2: 8 (pp15). 2015.

*. Hisaya Kojima, Yoshitoshi Ogura, Nozomi Yamamoto, Tomoaki Togashi, Hiroshi Mori, Tomohiro Watanabe, Fumiko Nemoto, Ken Kurokawa, Tetsuya Hayashi and Manabu Fukui. Ecophysiology of Thioploca ingrica as revealed by the complete genome sequence supplemented with proteomic evidence. The ISME Journal 9: 1166-1176.2015.

【2014年】
*. Hisaya Kojima, Riho Tokizawa and Manabu Fukui. Mizugakiibacter sediminis gen. nov., sp. nov., isolated from a freshwater lake. International Journal of Systematic and Evolutionary Microbiology 64: 3983-3987. 2014.

*. Hisaya Kojima, Riho Tokizawa, Kouhei Kogure, Yuki Kobayashi, Masayuki Itoh, Fuh-Kuw Shiah, Noboru Okuda and Manabu Fukui. Community structure of planktonic methane-oxidizing bacteria in a subtropical reservoir characterized by dominance of phylotype closely related to nitrite reducer. Scientific Reports 4: 5728. DOI: 10.1038/srep05728.

*. Tomohiro Watanabe, Hisaya Kojima, and Manabu Fukui. Complete genomes of freshwater sulfur oxidizers Sulfuricella denitrificans skB26 and Sulfuritalea hydrogenivorans sk43H: Genetic insights into the sulfur oxidation pathway of betaproteobacteria. Systematic and Applied Microbiology 37: 387-395.2014.

*. Miho Watanabe, Hisaya Kojima and Manabu Fukui. Proposal of Effusibacillus lacus gen. nov., sp. nov. and reclassification of Alicyclobacillus pohliae as Effusibacillus pohliae comb. nov. and Alicyclobacillus consociatus as Effusibacillus consociatus comb. nov.. International Journal of Systematic and Evolutionary Microbiology 64: 2770-2774.2014.

*. Kyoko Kubo, Hisaya Kojima, and Manabu Fukui. Vertical distribution of major sulfate-reducing bacteria in a eutrophic shallow meromictic lake. Systematic and Applied Microbiology 37: 510-519. 2014.

*. Hisaya Kojima, Johanna Moll, Joerg Kahnt, Manabu Fukui and Seigo Shima. A reversed genetic approach reveals the coenzyme specificity and the catalytic properties of three enzymes putatively involved in anaerobic oxidation of methane with sulfate. Environmental Microbiology 16: 3431-3442. 2014.

*. Hisaya Kojima, Tomohiro Watanabe, Tomoya Iwata and Manabu Fukui. Identification of major planktonic sulfur oxidizers in stratified freshwater lake. PLoS ONE : e93877. 2014.

*. Hisaya Kojima and Manabu Fukui. Sulfurisoma sediminicola gen. nov., sp. nov., a novel facultative autotroph isolated from a freshwater lake. International Journal of Systematic and Evolutionary Microbiology 64: 1587-1592. 2014.

【2013年】

*. Tomohiro Watanabe, Hisaya Kojima, Yoshinori Takano and Manabu Fukui. Diversity of sulfur-cycle prokaryotes in freshwater lake sediments investigated using aprA as functional marker gene. Systematic and Applied Microbiology 36: 436-443.2013.

*. Yoshinori Iizuka, Barbara Delmonte, Ikumi Oyabu, Torbjoern Karlin, Valter Maggi, Amuel Albani, Manabu Fukui, Takeo Hondoh and Margareta Hansson. Sulphate and chloride aerosols during Holocene and last glacial periods preserved in the Talos Dome Ice Core, a peripheral region of Antarctica. Tellus B 65: 20197.2013.

*. Miho Watanabe, Hisaya Kojima and Manabu Fukui. Desulfotomaculum intricatum sp. nov., a sulfate-reducer isolated from freshwater lake sediment. International Journal of Systematic and Evolutionary Microbiology 63: 3574-3578. 2013.

*.Takato Matsui, Hisaya Kojima, and Manabu Fukui. Effects of temperature on successive anaerobic decomposition of high-molecular organic matter under sulfate-reducing conditions. Estuarine, Coastal and Shelf Science 119: 139-144. 2013.

*. Akiko Kamono, Marianne Meyer, Thomas Cavalier-Smith,　Manabu Fukui and Anne Maria Fiore-Donno. Exploring slime mould diversity in high altitude forests and grasslands by environmental RNA analysis. FEMS Microbiology Ecology 84: 98-108. 2013.

*. Yuriko Higashioka, Hisaya Kojima, Miho Watanabe and Manabu Fukui. Desulfatitalea tepidiphila gen. nov., sp. nov., a novel sulfate-reducing bacterium isolated from tidal flat sediment. International Journal of Systematic and Evolutionary Microbiology 63: 761-765. 2013.

【2012年】

*. Yoshinori Takano, Jonathan James Tyler, Hisaya Kojima, Yusuke Yokoyama, Yukiko Tanabe, Takaharu Sato, Nanako O. Ogawa, Naohiko Ohkouchi, and Manabu Fukui. Holocene lake developments and glacial-isostatic uplift at Lake Skallen and Lake Oyako, Luetzow-Holm Bay, east Antarctica: based on biogeochemical facies and molecular signatures. Applied Geochemistry 27: 2546-2559. 2012.

*. Tomohiro Watanabe, Hisaya Kojima, and Manabu Fukui. Draft genome sequence of a psychrotolerant sulfur-oxidizing bacterium Sulfuricella denitrificans skB26 and proteomic insights into cold adaptation. Applied and Environmental Microbiology 78: 6545-6549. 2012.

*. Fumiko Nemoto, Hisaya Kojima, Akifumi Ohtaka and Manabu Fukui. Filamentous sulfur-oxidizing bacteria of the genus Thioploca from Lake Tonle Sap in Cambodia. Aquatic Microbial Ecology 66: 295-300. 2012.

*. Anne Maria Fiore-Donno, Akiko Kamono, Marianne Meyer, Martin Schnittler, Manabu Fukui and Thomas Cavalier-Smith. 18S rDNA phylogeny of Lanmproderma and allied genera (Stemonitales, Myxomycetes, Amoebozoa). PLoS ONE 7: e35359. 2012.

*. Hisaya Kojima, Masazumi Tsutsumi, Kanako Ishikawa, Tomoya Iwata, Marc Mussmann and Manabu Fukui. Distribution of putative denitrifying methane oxidizing bacteria of a freshwater lake. Systematic and Applied Microbiology 35: 233-238. 2012.

*. Yuriko Higashioka, Hisaya Kojima, and Manabu Fukui. Isolation and characterization of novel sulfate-reducing bacterium capable anaerobic degradation of p-xylene. Microbes and Environments 27: 273-277. 2012.

*. Masanaori Fujii, Hisaya Kojima, Tomoya Iwata, Jotaro Urabe and Manabu Fukui. Dissolved organic carbon as major environmental factor affecting bacterioplankton communities in mountain lakes of eastern Japan. Microbial Ecology 63: 496-508. 2012.

*. Masazumi Tsutsumi, Hisaya Kojima, and Manabu Fukui. Vertical profiles of abundance and potential activity of methane-oxidizing bacteria in sediment of Lake Biwa, Japan. Microbes and Environments 27: 67-71. 2012.

【2011年】

*. Fumiko Nemoto, Hisaya Kojima, and Manabu Fukui. Diversity of freshwater Thioploca species and their specific association with filamentous bacteria of the phylum Chloroflexi. Microbial Ecology 62：753-764.2011.

*. Hisaya Kojima, and Manabu Fukui. Sulfuritalea hydrogenivorans gen. nov., sp. nov., a novel facultative autotroph isolated from a freshwater lake. International Journal of Systematic and Evolutionary Microbiology 61: 1651-1655. 2011.

*. Masazumi Tsutsumi, Tomoya Iwata, Hisaya Kojima, and Manabu Fukui. Spatiotemporal variations in an assemblage of closely related planktonic aerobic methanotrophs. Freshwater Biology 56: 342-351. 2011.

*. Yuriko Higashioka, Hisaya Kojima and Manabu Fukui. Temperature-dependent differences in community structure of bacteria involved in degradation of petroleum hydrocarbons under sulfate-reducing conditions. Journal of Applied and Microbiology　110: 314-322. 2011.

*. Kyoko Kubo, Katrin Knittel, Rudolf Amann, Manabu Fukui and Katsumi Matsuura. Sulfur-metabolizing bacteria populations in microbial mats of the Nakabusa hot spring, Japan. Systematic and Applied Microbiology 34: 293-302, 2011.

【2010年】

*. Hisaya Kojima, and Manabu Fukui. Sulfuricella denitrificans gen. nov., sp. nov., a novel sulfur-oxidizing autotroph isolated from a freshwater lake. International Journal of Systematic and Evolutionary Microbiology 60:2862-2866. 2010.

*. Keiichi Tabuchi, Hisaya Kojima, and Manabu Fukui. Seasonal changes in organic matter mineralization in a sublittoral sediment and temperature-driven decoupling of key processes. Microbial Ecology 60: 551-560. 2010.

*. Masanaori Fujii, Yoshinori Takano, Hisaya Kojima, Tamotsu Hoshino, Ryoichi Tanaka and Manabu Fukui. Microbial community structure, pigment composition, and nitrogen source of red snow in Antarctica. Microbial Ecology 59: 466-4775.2010.

*. Anne Fiore-Donno, Akiko Kamono, Ema Chao, Manabu Fukui and Thomas Cavalier-Smith. Invalidation of hyperamoeba by transferring its species to other genera of Myxogastria. Journal of Eukaryotic Biology 52: 189-196. 2010.

【2009年】

*. Akiko Kamono, Hisaya Kojima, Jun Matsumoto, Kimitaka Kawamura and Manabu Fukui. Airborne Myxomycete spore: Detection using molecular techniques. Naturwissenschaften　96: 147-151.2009.

*. Masazumi Tsutsumi, Hisaya Kojima, Shigeru Uemura, Kiyomi Ono, Akihiro Sumida, Toshihiko Hara and Manabu Fukui. Structure and activity of soil-inhabiting methanotrophic communities in northern forest of Japan. Soil Biology and Biochemistry　 41: 403-408.2009.

*. Yuriko Higashioka, Hisaya Kojima, Tatsunori Nakagawa, Shinya Sato and Manabu Fukui. A novel n-alkane-degrading bacterium as a minor member of p-xylene-degrading sulfate-reducing consortium. Biodegradation　20: 383-390.2009.　

*. Yuriko Higashioka, Hisaya Kojima, Shinya Sato, Manabu Fukui. Microbial community analysis at crude oil-contaminated soils targeting the 16S ribosomal RNA, xylM, C23O, and bcr genes. Journal of Applied Microbiology　107: 126-135. 2009.

*. Hisaya Kojima, Tomoya Iwata, and Manabu Fukui. DNA-based analysis of planktonic methanotrophs in a stratified lake. Freshwater Biology　54: 1501-1509.2009.

*. Akiko Kamono, Jun Matsumoto, Hisaya Kojima, and Manabu Fukui. Characterization of myxomycete communities in soil by reverse transcription polymerase chain reaction (RT-PCR)-based method. Soil Biology and Biochemistry 41: 1324-1330. 2009.

*. Hisaya Kojima, Haruo Fukuhara and Manabu Fukui. Community structure of microorganisms asssociated with reddish-brown iron-rich snow. Systematic and Applied Microbiology 32: 429-437. 2009.