WO2011038114(特表2013-510559)
"12. A method for engineering an enzyme for use in detection of a substance of interest(目的物質), said method comprising:
creating a mutation at a residue that participates in the catalytic function of the enzyme for a chosen substrate to reduce or abolish the catalytic activity of the enzyme for that substrate, wherein the catalytic activity of the mutant enzyme for that substrate can be restored by the substance of interest; and
creating another mutation in the enzyme, wherein the other mutation increases the catalytic activity and specificity of the mutant enzyme for a pre-selected substrate in the presence of(存在下で)the substance of interest."
WO0185952(特表2004-514409)
"36. A method for identifying an agent of interest(目的物質)that inhibits the activity of a protein multimer comprising modified IMPDH polypeptides, the method comprising: a) contacting the protein multimer with(接触させる)inosine-5'-monophosphate, nicotinamide adenine dinucleotide, and the agent of interest ; and b) detecting the level of the reduced form of inosine-5'-monophosphate or nicotinamide adenine dinucleotide which is generated, whereby a low level of the reduced form of inosine-5'-monophosphate or nicotinamide adenine dinucleotide generated indicates that the agent of interest inhibits IMPDH activity."
WO2015200657(特表2017-528537)
"The crude heptafluorobutyl methacrylate was added to a 3 liter flask fitted with a distillation head and a thermocouple. More inhibitor (3 grams of phenothiazine and 0.7 gram of MEHQ) were added to the distillation pot. The acrylate was distilled to give 156 of precut distilling at 142 mm Hg at a head temperature of 80 °C-86 °C (88% desired methacrylate). The desired material(目的物質)distilled at 86 °C-°C at 131 mm Hg; a total of 1934 grams of heptafluorobutyl methacrylate were obtained."
WO2014113571(特表2016-503663)
"As used herein, the term fermentation broadly refers to the conversion of organic materials into target substances(目的物質)by host cells, for example, the conversion of a carbon source by recombinant host cells into fatty acids or derivatives thereof by propagating a culture of the recombinant host cells in a media comprising the carbon source."
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