アビジンとビオチン類の１：１複合アビジンとビオチン類（A-B錯体:図1）は多点相互作用の典型例で、極めて安定な複合体(解離定数10-15M)である。したがって、生化学標識法に汎く応用されているが、その生理機能はほとんど分かっていないのが実情である。当蛋白と基質は主に水素結合によりむすばれている。
図１ アビジン(蛋白)とビオチン(ビタミン)の１：１複合体（A-B錯体）の模式図。ESR免疫測定法、生化学標識法等に応用されている。
当複合体の結晶構造データは100以上に及んでおり、また、基質のビオチン誘導体も10種以上あり、当蛋白－基質相互作用の理論的解析は蛋白機能解明の格好の基盤となる。PDBより結晶構造データをファイルでダウンロードし、CASheを用いてHOF,その他の計算をする。
以下に、計算の手順とその結果を示す。
1) PDBデータをCACheデータに変換
2) 基質周りの３A又は４Å以内の原子種(またはアミノ酸残基）を選択、電荷の補正
3) 当該原子種(またはアミノ酸残基）を含むアミノ酸を抽出してポケットのモデルとして採用
4) 原子座標を固定化後、水素原子を修飾
5) AM1法MOPACで水素原子位置の最適化
6) AM1MOPACを用いて複合体のHOFを計算
7) 構造を固定したままで基質、ポケットをそれぞれ計算(水素原子はUNLOCK)
8) 複合体のHOFとの差をとって結合エネルギーを求める。
9) 基質分子情報として活性化エネルギー、HOMO、LUMO等を求める
表1 アビジンービオチン複合体の結合エネルギー
(クリックで拡大)
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