【論】Yang,2001,Normalization for cDNA microarray ～

Yee Hwa Yang, Sandrine Dudoit, Percy Luu, David M. Lin, Vivian Peng, John Ngai and Terence P. Speed
Normalization for cDNA microarray data: a robust composite method addressing single and multiple slide systematic variation
Nucleic Acids Research,2002, Vol.30, No.4 e15
[PDF][Web Site]

・マイクロアレイデータの正規化法の提案。サンプル内だけでなく、サンプル間や実験間のデータのばらつきも考慮に入れた、より普遍的な正規化法について。
・データ
A: apolopoprotein AI (apo AI) experiment, マウス, 16サンプル, 6384遺伝子 [Callow]
B: olfactory bulb experiment, マウス, 18000遺伝子 [RIKEN]
・比較した(サンプル内(within-slide))正規化法
1.Global median normalization
2.Intensity-dependent location normalization, "lowess"
3.Within-print tip group location normalization
4.Within-print tip group scale normalization

・問題点「However, such global normalization approaches are not adequate in situations where dye biases can depend on spot overall intensity and/or spatial location within the array.」
・方法「In this article we propose a composite normalization procedure, based on robust local regression, to accommodate different types of dye biases and the use of control sequences spotted on the array.」