この論文は引用元の明示なく他者の論文の文章を使用。剽窃を示唆するが引用元を 書き忘れた可能性もある。赤色が重複部分。「2006年にMerck Milliporeに買収されたChemicon社のURLや同社の古い試薬名（CpGenome DNA modification kit）までコピペしていたことが指摘されています。この試薬を使ったDNAメチル化の評価実験は、実際には行われていなかったのではないか？と疑われて います。」（リンク先からの引用）
→（調査結果）この部分は必然的によく似た記述になるとし剽窃とせず。試薬名の件は言及せず。
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Nature Article（☆2）
Bisulphite sequencing
GFP-positive cells in STAP clusters were collected by FACS Aria. Genomic DNA was extracted from STAP cells and analysed. Bisulphite treatment of DNA was performed using the CpGenome DNA modification kit (Chemicon, http://www.chemicon.com), following the manufacturer’s instructions. The resulting modified DNA was amplified by nested PCR using two forward (F) primers and one reverse (R) primer: Oct4 (F1, 5′-GTTGTTTTGTTTTGGTTTTGGATAT-3′; F2, 5′-ATGGGTTGAAATATTGGGTTTATTTA-3′; R, 5′-CCACCCTCTAACCTTAACCTCTAAC-3′). And Nanog (F1, 5′-GAGGATGTTTTTTAAGTTTTTTTT-3′; F2, 5′-AATGTTTATGGTGGATTTTGTAGGT-3′; R, 5′-CCCACACTCATATCAATATAATAAC-3′). PCR was done using TaKaKa Ex Taq Hot Start Version (RR030A). DNA sequencing was performed using a M13 primer at the Genome Resource and Analysis Unit, RIKEN CDB.
引用元、
Stem Cells. 2006 Sep;24(9):2007-13. Epub 2006 May 18.
Northern and Bisulfite Sequencing
For Northern analysis, 10 μg per sample of total RNA isolated using Trizol (Invitrogen, Carlsbad, CA, http://www.invitrogen.com) was run in each lane and then transferred to Gene Screen (PerkinElmer Life and Analytical Sciences, Boston, http://www.perkinelmer.com). The resulting membranes were probed using cDNAs spanning the open reading frames of the corresponding genes and washed under high stringency conditions (0.2× standard saline citrate, 65°C for 45 minutes) before exposure to film. Bisulfite treatment of DNA was done using the CpGenome DNA Modification Kit (Chemicon, Temecula, CA, http://www.chemicon.com) following the manufacturer's instructions. The resulting modified DNA was amplified by nested polymerase chain reaction (PCR) using two forward (F) primers and one reverse (R) primer: Oct4 (F1, GTTGTTTTGTTTTGGTTTTGGATAT; F2, ATGGGTTGAAATATTGGGTTTATTTA; R, CCACCCTCTAACCTTAACCTCTAAC) and Nanog (F1, GAGGATGTTTTTTAAGTTTTTTTT; F2, AATGTTTATGGTGGATTTTGTAGGT; R, CCCACACTCATATCAATATAATAAC). The first round of PCR was done as follows: 94°C for 4 minutes; five cycles of 94°C for 30 seconds, 56°C for 1 minute (−1°C per cycle), 72°C for 1 minute; and 30 cycles of 94°C for 30 seconds, 51°C for 45 seconds, and 72°C for 1 minute, 20 seconds. The second round of PCR was 94°C for 4 minutes; 30 cycles of 94°C for 30 seconds, 53.5°C for 1 minute, and 72°C for 1 minute 20 seconds. The resulting amplified products were gel-purified (Zymogen, Zymo Research, Orange, CA, http://www.zymoresearch.com), subcloned into the TOPO TA vector (Invitrogen), and sequenced using the M13F&R primers.
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（２－４）は11jigenのまとめブログを参考にした。
