David Stoddart, Andrew J. Heron, Ellina Mikhailova, Giovanni Maglia and Hagan Bayley.
PNAS, Early edition | doi:10.1073/pnas.0901054106
The sequencing of individual DNA strands with nanopores is under investigation as a rapid, low-cost platform in which bases are identified in order as the DNA strand is transported through a pore under an electrical potential. Although the preparation of solid-state nanopores is improving, biological nanopores, such as α-hemolysin (αHL), are advantageous because they can be precisely manipulated by genetic modification. Here, we show that the transmembrane β- barrel of an engineered αHL pore contains 3 recognition sites that can be used to identify all 4 DNA bases in an immobilized single-stranded DNA molecule, whether they are located in an otherwise homopolymeric DNA strand or in a heteropolymeric strand. The additional steps required to enable nanopore DNA sequencing are outlined.# 以前発表されたProof-of-concept paperとは異なり、ポリヌクレオチドの塩基の並びの違いを検出しようとする試み。膜貫通β-バレルをいじって三カ所の認識部位を作ってこれによって四種類の塩基を認識しようとしている。
