# 論文が見つからないものは、見つかるか諦めるまで掲載しないことにしてみるテスト
asahi.com： エボラウイルスの無毒化に成功 東大グループ - サイエンス
エボラ出血熱の原因ウイルス、東大チームが無害化に成功 : 科学 : YOMIURI ONLINE（読売新聞）
Generation of biologically contained Ebola viruses.
Peter Halfmann, Jin Hyun Kim, Hideki Ebihara, Takeshi Noda, Gabriele Neumann, Heinz Feldmann, Yoshihiro Kawaoka.
Proc. Natl. Acad. Sci. USA, Edition 10.1073/pnas.0708057105
Biotechnology Japan | 慶応大、三菱化学生命研、DNA断片を順番どおりつなぎ合わせ巨大ゲノムの構築に成功
慶應義塾大学先端生命科学研究所 (Institute for Advanced Biosciences, Keio University) - 遺伝子をつなぎ合わせてゲノムを構築
Life from information.
Christopher A Voigt.
Nature Methods 5, 27-28 (2008) | doi:10.1038/nmeth0108-27
Bottom-up genome assembly using the Bacillus subtilis genome vector.
Mitsuhiro Itaya, Kyoko Fujita, Azusa Kuroki, Kenji Tsuge.
Nature Methods 5, 41-43 (2007) | doi:10.1038/nmeth1143
asahi.com：医学部教授、９割が企業から寄付金 厚労省調査 - 社会
# だったら企業寄付金を全面禁止してその分を公的資金で補填するのかと…
asahi.com： 地球の内部は「乾いていた」 マントルほとんど水分なし - サイエンス
Dry mantle transition zone inferred from the conductivity of wadsleyite and ringwoodite.
Takashi Yoshino, Geeth Manthilake, Takuya Matsuzaki, Tomoo Katsura.
Nature 451, 326-329 (2008) | doi:10.1038/nature06427
「道に迷ったの？」大阪で道案内ロボットの実験 : 科学 : YOMIURI ONLINE（読売新聞）
# 余計なお世話になりそうなことはロボットにさせるという発想…
「しょっぱい」悩み解消 梅干し廃液で研究進む 和歌山 - MSN産経ニュース
# 食塩濃度が高いから希釈しないと処理は難しいわな
ＥＳ細胞を使い、筋ジス新治療法に成功 : ニュース : 医療と介護 : YOMIURI ONLINE（読売新聞）
米大チーム、マウスの筋ジストロフィー治療に成功、ES細胞移植 国際ニュース : AFPBB News
Functional skeletal muscle regeneration from differentiating embryonic stem cells.
Radbod Darabi, Kimberly Gehlbach, Robert M Bachoo, Shwetha Kamath, Mitsujiro Osawa, Kristine E Kamm, Michael Kyba, Rita C R Perlingeiro.
Nature Medicine, Advance online publication | doi:10.1038/nm1705
英米中の研究グループ、ゲノム情報１０００人分を解析へ : 科学 : YOMIURI ONLINE（読売新聞）
International genome project launched.
Erika Check Hayden.
Nature 451, 378-379 (2008) | doi:10.1038/451378b
# 1,000ドルゲノムのお次は1,000人ゲノム…
Peter Halfmann, Jin Hyun Kim, Hideki Ebihara, Takeshi Noda, Gabriele Neumann, Heinz Feldmann, Yoshihiro Kawaoka.
Proc. Natl. Acad. Sci. USA, Edition 10.1073/pnas.0708057105
Ebola virus (EBOV), a public health concern in Africa and a potential biological weapon, is classified as a biosafety level-4 agent because of its high mortality rate and the lack of approved vaccines and antivirals. Basic research into the mechanisms of EBOV pathogenicity and the development of effective countermeasures are restricted by the current biosafety classification of EBOVs. We therefore developed biologically contained EBOV that express a reporter gene instead of the VP30 gene, which encodes an essential transcription factor. A Vero cell line that stably expresses VP30 provides this essential protein in trans and biologically confines the virus to its complete replication cycle in this cell line. This complementation approach is highly efficient because biologically contained EBOVs lacking the VP30 gene grow to titers similar to those obtained with wild-type virus. Moreover, EBOVs lacking the VP30 gene are indistinguishable in their morphology from wild-type virus and are genetically stable, as determined by sequence analysis after seven serial passages in VP30-expressing Vero cells. We propose that this system provides a safe means to handle EBOV outside a biosafety level-4 facility and will stimulate critical studies on the EBOV life cycle as well as large-scale screening efforts for compounds with activity against this lethal virus.# この実験自体はP4で行ったんですよね？
Christopher A Voigt.
Nature Methods 5, 27-28 (2008) | doi:10.1038/nmeth0108-27
Mitsuhiro Itaya, Kyoko Fujita, Azusa Kuroki, Kenji Tsuge.
Nature Methods 5, 41-43 (2007) | doi:10.1038/nmeth1143
We established a protocol to construct complete recombinant genomes from their small contiguous DNA pieces and obtained the genomes of mouse mitochondrion and rice chloroplast using a B. subtilis genome (BGM) vector. This method allows the design of any recombinant genomes, valuable not only for fundamental research in systems biology and synthetic biology but also for various applications in the life sciences.# 相同組換えを繰り返して構築するようです
# だったら企業寄付金を全面禁止してその分を公的資金で補填するのかと…
Takashi Yoshino, Geeth Manthilake, Takuya Matsuzaki, Tomoo Katsura.
Nature 451, 326-329 (2008) | doi:10.1038/nature06427
The Earth's mantle transition zone could potentially store a large amount of water, as the minerals wadsleyite and ringwoodite incorporate a significant amount of water in their crystal structure. The water content in the transition zone can be estimated from the electrical conductivities of hydrous wadsleyite and ringwoodite, although such estimates depend on accurate knowledge of the two conduction mechanisms in these minerals (small polaron and proton conductions), which early studies have failed to distinguish between. Here we report the electrical conductivity of these two minerals obtained by high-pressure multi-anvil experiments. We found that the small polaron conductions of these minerals are substantially lower than previously estimated. The contributions of proton conduction are small at temperatures corresponding to the mantle transition zone and the conductivity of wadsleyite is considerably lower than that of ringwoodite for both mechanisms. The dry model mantle shows considerable conductivity jumps associated with the olivine–wadsleyite, wadsleyite–ringwoodite and post-spinel transitions. Such a dry model explains well the currently available conductivity–depth profiles obtained from geoelectromagnetic studies. We therefore conclude that there is no need to introduce a significant amount of water in the mantle transition to satisfy electrical conductivity constraints.# 電気伝導度だけでここまで言い切ってしまうところがある意味凄い…
# 余計なお世話になりそうなことはロボットにさせるという発想…
# 食塩濃度が高いから希釈しないと処理は難しいわな
Radbod Darabi, Kimberly Gehlbach, Robert M Bachoo, Shwetha Kamath, Mitsujiro Osawa, Kristine E Kamm, Michael Kyba, Rita C R Perlingeiro.
Nature Medicine, Advance online publication | doi:10.1038/nm1705
Little progress has been made toward the use of embryonic stem (ES) cells to study and isolate skeletal muscle progenitors. This is due to the paucity of paraxial mesoderm formation during embryoid body (EB) in vitro differentiation and to the lack of reliable identification and isolation criteria for skeletal muscle precursors. Here we show that expression of the transcription factor Pax3 during embryoid body differentiation enhances both paraxial mesoderm formation and the myogenic potential of the cells within this population. Transplantation of Pax3-induced cells results in teratomas, however, indicating the presence of residual undifferentiated cells. By sorting for the PDGF- receptor, a marker of paraxial mesoderm, and for the absence of Flk-1, a marker of lateral plate mesoderm, we derive a cell population from differentiating ES cell cultures that has substantial muscle regeneration potential. Intramuscular and systemic transplantation of these cells into dystrophic mice results in extensive engraftment of adult myofibers with enhanced contractile function without the formation of teratomas. These data demonstrate the therapeutic potential of ES cells in muscular dystrophy.# これってiPS細胞の方が適しているんじゃないかな
Erika Check Hayden.
Nature 451, 378-379 (2008) | doi:10.1038/451378b
# 1,000ドルゲノムのお次は1,000人ゲノム…
