# N末端21アミノ酸で十分な活性が得られるそうです
# もしそうだとしたら、既に疫学的にそういう傾向が見えているはず…
# 液体窒素じゃ低すぎるのか？
# これの結果が出たですね
# じゃぁ、スペースシャトルも紙で…というわけにはいきません
# おめでとうございます
# で、どのように注意しろと？
# 最悪だ…何とか回収して処理しないと衛星に影響しまくるぞ
# ミトコンドリアが牛で核がヒト…
# 2歳未満に使える市販薬って樋屋奇応丸や宇津救命丸みたいな生薬以外にあったんだ…
# これって、コンタクトレンズから網膜に直接像を結ぼうって魂胆ですか？
Jay Morris, Keli M. Hawthorne, Tim Hotze, Steven A. Abrams, Kendal D. Hirschi.
PNAS, Early Edition | doi:10.1073/pnas.0709005105
Nutrition recommendations worldwide emphasize ingestion of plant-based diets rather than diets that rely primarily on animal products. However, this plant-based diet could limit the intake of essential nutrients such as calcium. Osteoporosis is one of the world's most prevalent nutritional disorders, and inadequate dietary calcium is a known contributor to the pathophysiology of this condition. Previously, we have modified carrots to express increased levels of a plant calcium transporter (sCAX1), and these plants contain ~2-fold-higher calcium content in the edible portions of the carrots. However, it was unproven whether this change would increase the total amount of bioavailable calcium. In randomized trials, we labeled these modified carrots with isotopic calcium and fed them to mice and humans to assess calcium bioavailability. In mice feeding regimes (n = 120), we measured 45Ca incorporation into bones and determined that mice required twice the serving size of control carrots to obtain the calcium found in sCAX1 carrots. We used a dual-stable isotope method with 42Ca-labeled carrots and i.v. 46Ca to determine the absorption of calcium from these carrots in humans. In a cross-over study of 15 male and 15 female adults, we found that when people were fed sCAX1 and control carrots, total calcium absorption per 100 g of carrots was 41% ± 2% higher in sCAX1 carrots. Both the mice and human feeding studies demonstrate increased calcium absorption from sCAX1-expressing carrots compared with controls. These results demonstrate an alternative means of fortifying vegetables with bioavailable calcium.# あえてにんじんでカルシウムを摂ろうとは思わないんですけど…
Huichen Feng, Masahiro Shuda, Yuan Chang, Patrick S. Moore.
Science, Science Express | DOI: 10.1126/science.1152586
Merkel cell carcinoma (MCC) is a rare but aggressive human skin cancer that typically affects elderly and immunosuppressed individuals, a feature suggestive of an infectious origin. We studied MCC samples by digital transcriptome subtraction (DTS) and detected a fusion transcript between a previously undescribed virus T antigen and a human receptor tyrosine phosphatase. Further investigation led to discovery and sequence analysis of the 5387-base-pair genome of a new polyomavirus that we call Merkel cell polyomavirus (MCV or MCPyV). MCV sequences were detected in 8 of 10 (80%) MCC tumors but in only 5 of 59 (8%) control tissues from various body sites and 4 of 25 (16%) control skin tissues. In six of 8 MCV-positive MCCs, viral DNA was integrated within the tumor genome in a pattern suggesting that MCV infection/integration preceded clonal expansion of the tumor cells. Thus, MCV may be a contributing factor in the pathogenesis of MCC.# DTS (digital transcriptome subtraction)…憶えとこ…
Mari Ogawa, Hidefumi Shinohara, Youji Sakagami, Yoshikatsu Matsubayashi.
Science 319, 294 (2008) | DOI: 10.1126/science.1150083
CLV1, which encodes a leucine-rich repeat receptor kinase, and CLV3, which encodes a secreted peptide, function in the same genetic pathway to maintain stem cell populations in Arabidopsis shoot apical meristem. Here, we show biochemical evidence, by ligand binding assay and photoaffinity labeling, that the CLV3 peptide directly binds the CLV1 ectodomain with a dissociation constant of 17.5 nM. The CLV1 ectodomain also interacts with the structurally related CLE peptides, with distinct affinities depending on the specific amino acid sequence. Our results provide direct evidence that CLV3 and CLV1 function as a ligand-receptor pair involved in stem cell maintenance.幹細胞維持に関わるリガンドとレセプターとして働くそうな
# BlastocystからES細胞を取り出すところが相当難しいみたいですね
# こういう地道な実験が大事なんです
