Diagnostic performance of microscopic stool examination in Campylobacter infection performed by different medical specialties,
Journal of General and Family Medicine, 2022
廣瀬先生と明石先生、技師さんの力作です！
お疲れ様でした。
・便グラム染色検査のカンピロバクター検出に対する感度・特異度
医師A: 53.5% and 98.1%
医師B: 46.7% and 96.2%
検査技師：63.0% and 100%
臨床微生物検査技師：67.4% and 100%
抗原検査：感度：93.3%、特異度：99.0%.
Background
Microscopic examination of stool samples can contribute to the early diagnosis of Campylobacter gastroenteritis. However, it is unclear whether the diagnostic performance is reliable when performed by physicians.
Methods
This prospective study included fresh stool samples collected from patients with gastroenteritis between August 2018 and March 2020. The samples were used for microscopic examination through Gram staining. Two physicians, a clinical laboratory technician, and microbiologists performed the examinations. In addition, antigen tests (QuickNavi-Campylobacter; Denka Co., Ltd.) were evaluated for the samples collected between May 2019 and March 2020. Infection with Campylobacter spp. was confirmed when stool cultures or polymerase chain reaction tests provided positive results.
Results
Microscopic examination was performed on 205 samples, of which 46 (22.4%) were positive for Campylobacter spp. For the microscopic examination, the sensitivity and specificity were 53.5% and 98.1% for physician A, 46.7% and 96.2% for physician B, 63.0% and 100% for the clinical laboratory technician, and 67.4% and 100% for microbiologists, respectively. The antigen testing was evaluated in 131 of the 205 samples and showed a sensitivity of 93.3% and specificity of 99.0%.
Conclusions
Microscopic examination of the stool samples showed high specificity. The sensitivity when the examinations were performed by the physicians was insufficient. The rapid antigen tests can reliably detect Campylobacter spp. in stool samples.
