WEIHUAは、オリジナルの単層トップロール発泡機から20を超える一連の成形鋼生産ラインを開発しました。隠された屋根コイル成形機は、建設業界のニーズを満たすことができます。
隠された屋根コイル成形機
隠された屋根は、1990年代に世界で最も人気のあるルーフパネルで、バックルと自動ロックファスナーが端に固定されていました。
屋根の外側にネジはありません。設置は迅速かつ簡単で、断面特性が良好で、耐用年数は2倍以上長くなっています。
全く漏れがなく、あらゆる屋根に適しています。湾曲したロール成形機と協力して、スケルトン屋根システムを生産することができます。
ロール成形機の技術的パラメータ：
1.手動巻き戻し機：手動巻き出し、パッシブ拡張
2.アンコイラーの内径：508mm、610mm
3.アンコイラー容量：5T
4.フレーム：鋼構造の溶接、溶接後のショットブラストクリーニング、内部応力の除去
5.成形機のモーター力：7.5KW
6.モーター力の油圧場所：3KW
応用：
酵素生産
SSFでは、農業の工業用基質は酵素生産に最も優れていると考えられています。
SmFによる酵素生産の支出は、SSFと比較して高くなります。
およそ、すべてのよく知られている微生物の酵素はこのプロセスを通じて生産されます。研究によると、セルラーゼ、リパーゼ、プロテアーゼ、グルコアミラーゼ、アミラーゼ、リグニナーゼ、キシラナーゼ、ペクチナーゼ、ペルオキシダーゼなど、工業的に重要な酵素の生産に多大な労力が費やされています。
好熱性バチルスリケニホルミスによる耐熱性酵素キシラナーゼは、このプロセスから生産されました。このプロセスで生成される酵素は、SmFプロセスよりも熱安定性が高いです。 SSFシステムではSmFシステムよりも22倍高くなります。
オープンキシラン寒天プレートから抽出された菌株は、両方のプロセス（液中およびSSF発酵）のBacillus pumilusから生成されたキシラナーゼとして特徴付けられます。 Rhizopus oligosporusは米ぬかから酸性プロテアーゼを生産するために使用され、その生産中にSSFで毒素の影響は発生しませんでした。
The difference between PCR cloningand traditional cloning is that it can amplify target DNA fragments, or even vectors, by polymerase chain reaction (PCR) and ligate them together without the use of restriction enzymes.
PCR cloning is a fast method of cloning genes, usually used for projects that require higher throughput, and the throughput of these projects is higher than that of traditional cloning methods. It allows cloning of DNA fragments that are not available in large quantities.
Usually, a PCR reaction is performed to amplify the sequence of interest, and then it is connected to the vector by blunt-end or single-base overhang ligation before transformation.
The early PCR cloning of Taq DNA polymerase is often used to amplify the gene.
This results in a PCR product in which a single adenine (A) residue is added to the 3'end of the PCR product, which is independent of the template, through the normal action of the polymerase.
These "A-tail" products are then ligated to a complementary T-tail vector using T4 DNA ligase, and then transformed.
Now, high-fidelity DNA polymerases are also commonly used to amplify sequences that do not contain 3'extension PCR products.
The blunt-ended fragment is ligated to the plasmid vector by a typical ligation reaction or by the action of an "activated" vector containing a covalently ligated enzyme (usually topoisomerase I), which facilitates the vector:insert ligation.
Some PCR cloning systems contain engineered "suicide" vectors that include a toxic gene to which the PCR product must be successfully ligated to allow the propagation of strains that absorb the recombinant molecule during the transformation process.
A typical shortcoming shared by many PCR cloning methods is that special vectors must be used.
These vectors are usually sold by vendors (such as NEB) in ready-to-use linearized formats and can add significant costs to the total cost of cloning.
Likewise, the use of specific vectors limits the researchers' choices for antibiotic resistance, promoter identity, fusion partners and other regulatory elements.
✧ Efficient, with dedicated carrier
✧ Suitable for high throughput
✧ Limited vector selection
✧ higher cost
✧ Lack of sequence control at the junction
✧ Multi-fragment cloning is not easy
✧ Directional cloning is difficult
To study the desired functionality of an insert there is a requirement of transferring them from one vector to another, this procedure of transferring is known as subcloning. Steps of the Subcloning:
For any work on subcloning some prerequisite knowledge about the vector and insert is necessary.
The information about the RE sites available in the parent vector multiple cloning regions, RE sites available the insert (in case of inset digestion). Also information about the recurrence of RE sites in destination vector multiple cloning regions and RE sites in the insert.
Depending on the different combination of these sites, the subcloning strategy to be used is decided.
A straightforward subcloning process can be utilized if the parent and destination vector multiple cloning regions contain common restriction sites and neither of these restriction sites occurs within your insert.
Both the parent and destination vectors are digested using same two enzymes. This is followed by dephosphorylation of the destination vector. Separate both the insert and the dephosphorylated vector using an agarose gel. Then purify insert vector using any DNA purification system. Finally, these vectors and inserts are ligated.
There are cases when restriction sites in the parent and destination vector multiple cloning regions are not common but may be compatible. Compatible restriction sites have the same overhang sequence and can be ligated together.
After ligation, the regenerated sites does not resemble both restriction sites in the parent and destination vector multiple cloning regions. Once the necessary enzymes for the ligation and restriction are identified. This cloning strategy also becomes straightforward.
✦ Moving Inserts with Only One Common Site
The combination of common restriction sites leads to a possibility to only one match on one side of your insert. The issue should be addressed on the other side of the insert. A cut-blunt-cut can be used.
The action of T4 DNA Polymerase can blunt any restriction site. Digest the parent vector and blunt that site with T4 DNA Polymerase. Run the products on a gel, purify and proceed with the common or compatible end restriction enzyme digestion.
Most vectors have at least one blunt-ended restriction site that can accept the newly created blunt end from the insert.
If you don’t have such a site or the site would not be in the correct orientation, the same “cut-blunt-cut” strategy may be applied to the destination.
The final scenario of no common restriction site is common or compatible with the parent or destination vector.
IN this scenario the best strategy is to amplify the insert with restriction sites in the primers to provide the compatibility. But this method has an own set of problems.
There is a possibility of mutations. Also, there is a lot of difficulty in the digestion of the PCR products. One more strategy can be used in this scenario. Cut out the insert with any enzymes.
Treat with T4 DNA Polymerase to blunt either 5′ or 3′ overhangs and ligate into t
Inspection of cylinder block of Hitachi crawler excavator:
Remove water rust or other impurities on the surface of the cylinder block, remove the liquid sealant on the crankcase installation surface, and clean the cylinder block. Note: Do not damage the cylinder block.
Perform color inspection and hydraulic (pneumatic) testing. If cracks or other damage are found, replace the cylinder block.
(1) Check whether the inner surface of the cylinder liner is cracked or damaged.
(2) When the cylinder sleeve is in the cylinder block, measure the inner diameter of the cylinder liner at the most severely worn part at a distance of 15-20 mm from the top of the cylinder liner. If the wear exceeds the limit, replace the cylinder liner. Note: Since there is only one specification for the inner diameter of the cylinder liner, there is no need to select the inner diameter grade of the cylinder liner.
(1) Pull out the cylinder liner to remove the scale on the cylinder block.
(2) Use a ruler and a thickness gauge to measure the gap between the 4 sides and 2 diagonals on the upper surface of the cylinder block. Check the upper surface of the cylinder block. If the measured value exceeds the limit, replace the cylinder block.
If you are not very clear on how to inspect cylinder block of Hitachi crawler excavator, you can seek help from China Machinery Engineering Machinist.
