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The single Pro substitution on the cyanobacterial

2017-06-02 10:24:56 | 日記


In this study, we showed that a cyanobacterial ChlB variant (ChlB_PR) that mimics pre-edited plastid ChlB significantly decreases DPOR activity relative to wild-type ChlB. We also showed that the transcript levels of three plastid genes coding for DPOR subunits are up- and down-regulated in dark and light conditions, respectively, in seedlings of P. thunbergii. In addition, the RNA editing efficiency of chlB transcripts correlated well with transcript levels, being markedly higher in dark-grown than in light-grown seedlings. If the activity observed in the cyanobacterial ChlB variant is applicable to black pine chloroplast ChlB then this suggests that DPOR activity is also regulated at the post-transcriptional stage via RNA editing.

The single Pro substitution on the cyanobacterial ChlB (ChlB_P) at the same position as that which occurs by RNA editing of the eukaryotic homologs has a negative effect on formation of a complex with ChlN. In addition, this mutation also causes a decrease in solubility. These negative effects are also manifest by expression in E. coli cells in which no complex formation with ChlN was observed (Fig. 3,c). Interestingly, ChlB_P did complement Chl biosynthesis in chlB deletion strain (YFB14/NB2_P; Fig. 2B,a) indicating that this mutation can form enough of a functional NB complex in cyanobacterial cells to maintain Chl synthesize in the dark. These results also indicate that cyanobacterial cells may contain a stabilizing factor(s), such as Pchlide that allows Led Tube the ChlB Pro substitution to form a NB complex. The additional Arg substitution (ChlB_PR) caused more severe effect on complex formation with ChlN with this negative effect observed even in cyanobacterial cells.
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